Loss of Gramicidin Biosynthesis in Gram-Positive Biocontrol Bacterium Aneurinibacillus migulanus (Takagi et al., 1993) Shida et al. 1996 Emend Heyndrickx et al., 1997 Nagano Impairs Its Biological Control Ability of Phytophthora
نویسندگان
چکیده
The soil-borne species Aneurinibacillus migulanus (A. migulanus) strains Nagano and NCTC 7096 were shown to be potent biocontrol agents active against several plant diseases in agricultural forest ecosystems. Both produce the cyclic peptide gramicidin S (GS) that was described as main weapon inhibiting some gram-negative gram-positive bacteria fungus-like organisms along with production of biosurfactant hemolysis activities. However, contribution ability A. has never been studied experimentally. In this paper, using a mutant strain (E1 mutant) impaired GS biosynthesis we evaluated potential Phytophthora spp. two migulanus, 7096, tested pilot study for inhibition growth 13 dual culture assays. significantly more inhibitory than all species. Additionally, apple infection assays, P. rosacearum MKDF-148 cryptogea E2 most aggressive on fruits displaying clear halos. Therefore, three strains, Nagano, E1, used experiments check their effect these Treatment reduced severity symptoms compared 7096. E1 showed total loss suggesting is major actor strain.
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ژورنال
عنوان ژورنال: Forests
سال: 2022
ISSN: ['1999-4907']
DOI: https://doi.org/10.3390/f13040535